15115-1-AP

antibody from Invitrogen Antibodies

Targeting: HYAL2 LuCa-2, LUCA2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

15115-1-AP

Provider

Invitrogen Antibodies

Product name

HYAL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GVPQNVSLW AHRKMLQKRV EHYIRTQESA GLAVIDWEDW RPVWVRNWQD KDVYRRLSRQ LVASRHPDWP PDRIVKQAQY EFEFAAQQFM LETLRYVKAV RPRHLWGFYL FPDCYNHDYV QNWESYTGRC PDVEVARNDQ LAWLWAESTA LFPSVYLDET LASSRHGRNF VSFRVQEALR VARTHHANHA LPVYVFTRPT YSRRLTGLSE MDLISTIGES AALGAAGVIL WGDAGYTTST ETCQYLKDYL TRLLVPYVVN VSWATQYCSR AQCHGHGRCV RRNPSASTFL HLSTNSFRLV PGHAPGEPQL RPVGELSWAD IDHLQTHFRC QCYLGWSGEQ CQWDHRQAAG G (99-448 aa encoded by BC000692)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.63 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15115-1-AP (HYAL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using HYAL2 antibody 15115-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15115-1-AP (HYAL2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug HYAL2 antibody (15115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IGG with dilution 1:100.