PA5-31593

antibody from Invitrogen Antibodies

Targeting: SRRT ARS2, Asr2, serrate

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31593

Provider

Invitrogen Antibodies

Product name

ARS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, Jurkat, Raji, HeLa, HepG2. Predicted reactivity: Mouse (97%), Zebrafish (80%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.76 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.

Gebhardt A, Habjan M, Benda C, Meiler A, Haas DA, Hein MY, Mann A, Mann M, Habermann B, Pichlmair A
Nature communications 2015 Sep 18;6:8192

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ARS2 Polyclonal Antibody detects ARS2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. ARS2 Polyclonal Antibody (Product # PA5-31593) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ARS2 in paraformaldehyde-fixed A431 cells using an ARS2 polyclonal antibody (Product # PA5-31593) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ARS2 (Product # PA5-31593) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. ( a ) GFP-tagged NCBP3, NCBP2 or control protein (ctrl) stably expressed in HeLa cells were precipitated and associated proteins were analysed by LC-MS/MS. Volcano plots show the average degrees of enrichment by NCBP3 or NCBP2 over ctrl (ratio of LFQ protein intensities; x axis) and P values ( t -test; y axis) for each protein. Bait proteins (red letters), proteins of the exon junction complex (EJC, turquoise dots) or the TREX complex (purple dots), CBC (magenta dots), NELF (olive dots), importin (green dots) and NCBP3, SRRT and PHAX (red dots) are highlighted. Significantly enriched proteins are separated from the background by a hyperbolic curve. Four independent affinity purifications were performed for each bait. ( b ) Schematic illustration of AP-MS analysis in a focusing on proteins involved in RNA biogenesis. ( c ) Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. ( d ) Binding of NCBP1 to NCBP3 in E. coli . Western blot after His-precipitation using RNAse-treated lysates from bacteria co-expressing recombinant SII-tagged NCBP1 and full-length MBP/His-tagged NCBP3 or MBP/His as control. ( e ) NCBP2 and NCBP3 are required for NCBP1 association to m 7 GTP. Lysates of HeLa cells treated twice with siRNA against NCBP2, NCBP3 or both were used for m 7 GTP-affinity purification (m 7 GTP-AP), followed by western blot analysis. The m 7 GTP-b