PA5-109304

antibody from Invitrogen Antibodies

Targeting: MCU C10orf42, CCDC109A, FLJ46135

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109304

Provider

Invitrogen Antibodies

Product name

MCU Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A549, HL-60, mouse spleen tissue lysates, mouse brain tissue lysates, A431, HT-29, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, human colon tissue, human esophagus tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MCU in Lane 1: A549, Lane 3: mouse spleen tissue, Lane 2: HL-60, Lane 4: mouse brain tissue . Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCU in HT-29 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCU in HT-29 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCU in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCU in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MCU in paraffin-embedded rat cerebellum tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MCU in paraffin-embedded mouse brain tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MCU in paraffin-embedded human lung cancer tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MCU in paraffin-embedded human esophagus tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MCU in paraffin-embedded human colon tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of MCU in A549 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with MCU polyclonal antibody (Product # PA5-109304) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.