Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-109304 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCU Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A549, HL-60, mouse spleen tissue lysates, mouse brain tissue lysates, A431, HT-29, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, human colon tissue, human esophagus tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCU in Lane 1: A549, Lane 3: mouse spleen tissue, Lane 2: HL-60, Lane 4: mouse brain tissue . Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCU in HT-29 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCU in HT-29 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCU in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCU in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with MCU polyclonal antibody (Product # PA5-109304), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCU in paraffin-embedded rat cerebellum tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCU in paraffin-embedded mouse brain tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCU in paraffin-embedded human lung cancer tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCU in paraffin-embedded human esophagus tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MCU in paraffin-embedded human colon tissue. Samples were incubated with MCU polyclonal antibody (Product # PA5-109304), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MCU in A549 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with MCU polyclonal antibody (Product # PA5-109304) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.