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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 21059-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PNO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MESEMETQS ARAEEGFTQV TRKGGRRAKK RQAEQLSAAG EGGDAGRMDT EEARPAKRPV FPPLCGDGLL SGKEETRKIP VPANRYTPLK ENWMKIFTPI VEHLGLQIRF NLKSRNVEIR TCKETKDVSA LTKAADFVKA FILGFQVEDA LALIRLDDLF LESFEITDVK PLKGDHLSRA IGRIAGKGGK TKFTIENVTR TRIVLADVKV HILGSFQNIK MARTAICNLI LGNPPSKVYG NIRAVASRSA DRF (1-252 aa encoded by BC008304)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.14 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 21059-1-AP) (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PNO1 (IP:21059-1-AP, 3ug; Detection:21059-1-AP 1:300) with L02 cells lysate 1500ug.
