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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ABIN954150 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Phosphatidylinositol-4-Phosphate 5-Kinase-Like 1 (PIP5KL1) (N-Term), (AA 64-94) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 64-94 amino acids from the N-terminal region of human PIP5KL1
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 64-94
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Overexpression of PIP5KL1 suppresses cell proliferation and migration in human gastric cancer cells.
hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
Identification and characterization of a phosphoinositide phosphate kinase homolog.
Shi L, Zhao M, Luo Q, Ma YM, Zhong JL, Yuan XH, Huang CZ
Molecular biology reports 2010 Jun;37(5):2189-98
Molecular biology reports 2010 Jun;37(5):2189-98
hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes.
Lamesch P, Li N, Milstein S, Fan C, Hao T, Szabo G, Hu Z, Venkatesan K, Bethel G, Martin P, Rogers J, Lawlor S, McLaren S, Dricot A, Borick H, Cusick ME, Vandenhaute J, Dunham I, Hill DE, Vidal M
Genomics 2007 Mar;89(3):307-15
Genomics 2007 Mar;89(3):307-15
Identification and characterization of a phosphoinositide phosphate kinase homolog.
Chang JD, Field SJ, Rameh LE, Carpenter CL, Cantley LC
The Journal of biological chemistry 2004 Mar 19;279(12):11672-9
The Journal of biological chemistry 2004 Mar 19;279(12):11672-9
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of PIP5KL1 Antibody (N-term) Cat.-No AP53309PU-N in K562 cell line lysates (35ug/lane). This demonstrates the PIP5KL1 antibody detected the PIP5KL1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PIP5KL1 Antibody (N-term) Cat.-No AP53309PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle reacted with PIP5KL1 Antibody (N-term) Cat.-No AP53309PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
