MA5-20838

antibody from Invitrogen Antibodies

Targeting: GIT2 KIAA0148, PKL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-20838

Provider

Invitrogen Antibodies

Product name

GIT2 Monoclonal Antibody (M1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Peptide Sequence: MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDMYDEVD RRETDAVWLA TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL SDGPVTVQEF MEVKNALVAS EAKIQQLMKV NNNLSDELRI MQKKLLGKDA N

Antibody clone number

M1

Concentration

1.0 mg/mL

References

Submitted references

Surfactant Protein D Dampens Lung Injury by Suppressing NLRP3 Inflammasome Activation and NF-κB Signaling in Acute Pancreatitis.

Yu J, Ni L, Zhang X, Zhang J, Abdel-Razek O, Wang G
Shock (Augusta, Ga.) 2019 May;51(5):557-568

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GIT2 expressed in transfected 293T cell line by anti-GIT2 monoclonal antibody (Product # MA5-20838), clone M1. Lane 1: GIT2 transfected lysate (Predicted MW: 52.6 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using an anti-GIT2 monoclonal antibody (Product # MA5-20838). (antibody concentration 10 µg/mL).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of GIT2 transfected lysate using anti-GIT2 monoclonal antibody (Product # MA5-20838) and Protein A Magnetic Beads, and immunoblotted with GIT2 rabbit polyclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GIT2 using anti-GIT2 monoclonal antibody (Product # MA5-20838) is 1 ng/mL as a capture antibody.