12506-1-AP

antibody from Invitrogen Antibodies

Targeting: PLEK P47

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12506-1-AP

Provider

Invitrogen Antibodies

Product name

PLEK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEPKRIREG YLVKKGSVFN TWKPMWVVLL EDGIEFYKKK SDNSPKGMIP LKGSTLTSPC QDFGKRMFVF KITTTKQQDH FFQAAFLEER DAWVRDIKKA IKCIEGGQKF ARKSTRRSIR LPETIDLGAL YLSMKDTEKG IKELNLEKDK KIFNHCFTGN CVIDWLVSNQ SVRNRQEGLM IASSLLNEGY LQPAGDMSKS AVDGTAENPF LDNPDAFYYF PDSGFFCEEN SSDDDVILKE EFRGVIIKQG CLLKQGHRRK NWKVRKFILR EDPAYLHYYD PAGAEDPLGA IHLRGCVVTS VESNSNGRKS EEENLFEIIT ADEVHYFLQA ATPKERTEWI KAIQMASRTG K (1-350 aa encoded by BC018549)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U-937 cells were subjected to SDS PAGE followed by western blot with 12506-1-AP ( PLEK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PLEK antibody (12506-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat spleen tissue were subjected to SDS PAGE followed by western blot with 12506-1-AP (PLEK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 12506-1-AP (PLEK antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PLEK (IP: 12506-1-AP, 3 µg; Detection: 12506-1-AP 1:1000) with U-937 cells lysate 3000 µg.