66049-1-IG

antibody from Invitrogen Antibodies

Targeting: MMS19 CIAO4, hMMS19, MET18, MMS19L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66049-1-IG

Provider

Invitrogen Antibodies

Product name

MMS19 Monoclonal Antibody (2H6D8)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: FLDGNVSFL PENSFPSRFQ PFQDGSSGQR RLIALLMAFV CSLPRNVEIP QLNQLMRELL ELSCCHSCPF SSTAAAKCFA GLLNKHPAGQ QLDEFLQLAV DKVEAGLDSG PCRSQAFTLL LWVTKALVLR YHPLSSCLTA RLMGLLSDPE LGPAAADGFS LLMSDCTDVL TRAGHAEVRI MFRQRFFTDN VPALVQGFHA APQDVKPNYL KGLSHVLNRL PKPVLLPELP TLLSLLLEAL SCPDCVVQLS TLSCLQPLLL EAPQVMSLHV DTLVTKFLNL SSSPSMAVRI AALQCMHALT RLPTPVLLPY KPQVIRALAK PLDDKKRLVR KEAVSARGEW FLLGSPGS (457-803 aa encoded by BC006575)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2H6D8

Vial size

150 µL

Concentration

0.98 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66049-1-IG ( MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( ) fixed Hela cells using 66049-1-IG (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( ) fixed Hela cells using 66049-1-IG (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer using 66049-1-IG (MMS19 antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MMS19 (IP:66049-1-IG, 4ug; Detection:66049-1-IG 1:2000) with mouse brain tissue lysate 4000ug.