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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-47609 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47609, RRID:AB_10010566
- Product name
- Mouse Monoclonal Kv3.4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 70kDa (100kDa in brain due to glycosylation).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antigen sequence
(GDEAGDDERELALQRLGP)- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kv3.4 Antibody (S72-16) [NBP1-47609] - analysis of Rat brain membrane lysate showing detection of Kv3.4 Potassium Channel protein using Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody, Clone S72-16 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Kv3.4 Antibody (S72-16) [NBP1-47609] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli .
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Kv3.4 Antibody (S72-16) [NBP1-47609] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.4 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrinlike staining.
