18138-1-AP

antibody from Invitrogen Antibodies

Targeting: ABCD1 adrenoleukodystrophy, ALD, ALDP, AMN

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18138-1-AP

Provider

Invitrogen Antibodies

Product name

ABCD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SESDAEAVK KAALEKKEEE LVSERTEAFT IARNLLTAAA DAIERIMSSY KEVTELAGYT ARVHEMFQVF EDVQRCHFKR PRELEDAQAG SGTIGRSGVR VEGPLKIRGQ VVDVEQGIIC ENIPIVTPSG EVVVASLNIR VEEGMHLLIT GPNGCGKSSL FRILGGLWPT YGGVLYKPPP QRMFYIPQRP YMSVGSLRDQ VIYPDSVEDM QRKGYSEQDL EAILDVVHLH HILQREGGWE AMCDWKDVLS GGEKQRIGMA RMFYHRPKYA LLDECTSAVS IDVEGKIFQA AKDAGIALLS ITHRPSLWKY HTHLLQFDGE GGWKFEKLDS AARLSLTEEK QRLEQQLAGI PKMQRRLQEL CQILGEAVAP AHVPAPSPQG PGGLQGAST (358-745 aa encoded by BC025358)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were intracellularly stained with 0.4 µg Anti-Human ABCD1 (Product # 18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.4 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug.