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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ACC-102-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CACNA2D2 (CaV alpha 2 delta 2) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates: - 1-5. Anti-CACNA2D2 (CaV alpha 2 delta 2) (extracellular) Antibody (#ACC-102), (1:200).6-10. Anti-CACNA2D2 (CaV alpha 2 delta 2) (extracellular) Antibody , preincubated with CACNA2D2/Cav alpha 2 delta 2 (extracellular) Blocking Peptide (#BLP-CC102).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung (lanes 1 and 6), rat brain (lanes 2 and 7), mouse brain (lanes 3 and 8), rat heart (lanes 4 and 9) and mouse heart (lanes 5 and 10) lysates: - 1-5. Anti-CACNA2D2 (CaV alpha 2 delta 2) (extracellular) Antibody (#ACC-102), (1:200).6-10. Anti-CACNA2D2 (CaV alpha 2 delta 2) (extracellular) Antibody , preincubated with CACNA2D2/Cav alpha 2 delta 2 (extracellular) Blocking Peptide (#BLP-CC102).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of CaV alpha 2 delta 2 channel in rat cerebellum - Immunohistochemical staining rat cerebellum using Anti-CACNA2D2 (CaV alpha 2 delta 2) (extracellular) Antibody (#ACC-102). A. CaV alpha 2 delta 2 (green) appears in the soma of Purkinje cells (arrow) and in the molecular layer (M). B. Preincubation of the Antibody with the blocking peptide blocks staining of Purkinje cells and molecular layer. DAPI is used as the counterstain (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofCaV alpha 2 delta 2by indirect flow cytometry in live intacthumanJurkatT-cell leukemia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-CACNA2D2 (CaV alpha 2 delta 2) (extracellular) Antibody (#ACC-102), (5μg) + goat- Anti-rabbit-FITC.
