Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21563 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNFRSF19L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (87%), Rat (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references RELT stains prominently in B-cell lymphomas and binds the hematopoietic transcription factor MDFIC.
Cusick JK, Alhomsy Y, Wong S, Talbott G, Uversky VN, Hart C, Hejazi N, Jacobs AT, Shi Y
Biochemistry and biophysics reports 2020 Dec;24:100868
Biochemistry and biophysics reports 2020 Dec;24:100868
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TNFRSF19L Polyclonal Antibody (Product # PA5-21563). Sample (30 µg whole cell lysate). A: HeLa S3. 10% SDS PAGE. TNFRSF19L Polyclonal Antibody (Product # PA5-21563) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TNFRSF19L in methanol-fixed A431 cells using a TNFRSF19L polyclonal antibody (Product # PA5-21563) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using RELT antibody (Product # PA5-21563) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft , using RELT (Product # PA5-21563) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 RELT is expressed in human lymph nodes and B-cell lymphomas. Immunohistochemistry was performed on both normal lymph nodes and resected B-cell lymphomas using an antibody directed against RELT as described in Materials and Methods. (A) Comparison of RELT staining in normal lymph nodes, low-grade, and high-grade B-cell lymphomas. RELT staining at the indicated magnification with the presence of macrophages in normal lymph nodes indicated by red arrowheads. (B) RELT co-localizes with CD20 in B-cell lymphomas. Immunofluorescence was conducted with RELT and CD20 antibodies as described in Materials and Methods resulting in RELT fluorescing red, CD20 fluorescing green, and DAPI staining nuclei blue. Photographs were taken at 40x magnification. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Fig. 5