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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10012 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10012, RRID:AB_1674784
- Product name
- FBXO9 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FBXO9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Systematic analysis and nomenclature of mammalian F-box proteins.
A family of mammalian F-box proteins.
Antigens recognized by autologous antibody in patients with renal-cell carcinoma.
Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW
Genes & development 2004 Nov 1;18(21):2573-80
Genes & development 2004 Nov 1;18(21):2573-80
A family of mammalian F-box proteins.
Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW
Current biology : CB 1999 Oct 21;9(20):1180-2
Current biology : CB 1999 Oct 21;9(20):1180-2
Antigens recognized by autologous antibody in patients with renal-cell carcinoma.
Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jäger D, Jäger E, Knuth A, Chen YT, Old LJ
International journal of cancer 1999 Nov 12;83(4):456-64
International journal of cancer 1999 Nov 12;83(4):456-64
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using FBXO9 polyclonal antibody (Cat # PAB10012) shows detection of a band at ~100 KDa (arrowhead) believed to correspond to FBOX9 present in a MCF-7 whole cell lysate (Lane 1).Specific band reactivity is greatly diminished when theantibody is pre-incubated with the immunizing peptide (data not shown).Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,500.The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red).IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
