Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-23474 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FBXO9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FBXO9 in ThP1 cell lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in FBXO9 polyclonal antibody (Product # PA5-23474) using a dilution of 5 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of FBXO9 in HeLa cells. Samples were incubated in FBXO9 polyclonal antibody (Product # PA5-23474) using a dilution of 1:100 followed by DyLight 488-conjugated secondary antibody (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FBXO9 in formalin fixed paraffin embedded tissue section of human pancreas. Samples were incubated in FBXO9 polyclonal antibody (Product # PA5-23474) using a dilution of 1:100. The antibody generated a cytoplasmic-nuclear staining in cells of Islets of Langerhans while the pancreatic acini showed very mild to negligible signal for FBX09 immunostaining.