PAB27887

antibody from Abnova Corporation

Targeting: ANKRD2 ARPP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27887

Provider

Abnova Corporation

Product name

ANKRD2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ANKRD2.

Antigen sequence

IIKLLLLHGADMMTKNLAGKTPTDLVQLWQADTRH
ALEHPEPGAEHNGLEGPNDSGRETPQPVPAQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412545) with ANKRD2 polyclonal antibody (Cat # PAB27887) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with ANKRD2 polyclonal antibody (Cat # PAB27887) at 1-4 ug/mL dilution shows positivity in vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle with ANKRD2 polyclonal antibody (Cat # PAB27887) shows strong cytoplasmic positivity in myocytes at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)