H00007867-M01

antibody from Abnova Corporation

Targeting: MAPKAPK3 3pK, MAPKAP3, MK-3, MK3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007867-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007867-M01, RRID:AB_530119

Product name

MAPKAPK3 monoclonal antibody (M01), clone 3F4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant MAPKAPK3.

Antigen sequence

EVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQ
SMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALAT
MRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSAS
QGCNNQ


Isotype

IgG

Antibody clone number

3F4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MAPKAPK3 monoclonal antibody (M01), clone 3F4. Western Blot analysis of MAPKAPK3 expression in NIH/3T3 ( Cat # L018V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAPKAPK3 expression in transfected 293T cell line by MAPKAPK3 monoclonal antibody (M01), clone 3F4.Lane 1: MAPKAPK3 transfected lysate (Predicted MW: 43 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAPKAPK3 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of MAPKAPK3 transfected lysate using anti-MAPKAPK3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MAPKAPK3 monoclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol