OAAB09525

antibody from Aviva Systems Biology

Targeting: BNIP3 Nip3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

OAAB09525

Provider

Aviva Systems Biology

Product name

BNIP3 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

The anti-NIP3 BH3 domain Pab (Cat. #OAAB09525) is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).

Immunocytochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody Cat # OAAB09525. Data courtesy of Ruben Zamora, University of Pittsburgh.