Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [2]
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Validation data
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- Product number
- PA5-22961 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This CTR2 antibody is useful for Western blot, where a band is seen at ~71 kDa. The theoretical molecular weight of CTR2 is 15 kDa. The difference between the expected size and the observed size in Western blot is believed to be due to a multimeric form of CTR2.Suggested positive control: MEF whole cell lysate.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.93 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Copper-catalyzed dicarbonyl stress in NAFLD mice: protective effects of Oleuropein treatment on liver damage.
Copper/MYC/CTR1 interplay: a dangerous relationship in hepatocellular carcinoma.
Santini SJ, Tarantino G, Iezzi A, Alisi A, Balsano C
Nutrition & metabolism 2022 Feb 11;19(1):9
Nutrition & metabolism 2022 Feb 11;19(1):9
Copper/MYC/CTR1 interplay: a dangerous relationship in hepatocellular carcinoma.
Porcu C, Antonucci L, Barbaro B, Illi B, Nasi S, Martini M, Licata A, Miele L, Grieco A, Balsano C
Oncotarget 2018 Feb 6;9(10):9325-9343
Oncotarget 2018 Feb 6;9(10):9325-9343
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTR2 using a polyclonal antibody (Product # PA5-22961).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTR2 in 0.5 mg/mL MEF (left) and Human Placenta (right) lysate. Samples were incubated in CTR2 polyclonal antibody (Product # PA5-22961). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTR2 in MEF cell lysate. Sample was incubated in CTR2 polyclonal antibody (Product # PA5-22961).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CTR2 in 0.5 mg/mL MEF (left) and Human Placenta (right) lysate. Samples were incubated in CTR2 polyclonal antibody (Product # PA5-22961). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Effects of Ole Cu-transporter proteins in ND and HFD mice. RT-PCR, Western blot analysis and relative densitometry of CTR1 ( a ) and CTR2 ( b ) proteins. Values are expressed as fold mean +- SD. Data were analyzed by a factorial ANOVA with post-hoc Fisher's tests for multiple comparison (* P < 0.05, ** P < 0.01; *** P < 0.001 vs. ND mice; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. HFD mice; SS P < 0.05, SSSS P < 0.01 vs. male mice)