NBP2-04143

antibody from Novus Biologicals

Targeting: SRSF4 SFRS4, SRP75

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-04143

Provider

Novus Biologicals

Product name

Rabbit Polyclonal SFRS4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: SFRS4 Antibody [NBP2-04143] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRp75 antibody NBP2-04143 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp75 was also immunoprecipitated by rabbit anti-SRp75 antibody NBP2-04144, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: SFRS4 Antibody [NBP2-04143] - Samples: FFPE serial sections of human lung carcinoma. Antibody: Affinity purified rabbit anti- SRp75 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).