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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-04144 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SFRS4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SFRS4 Antibody [NBP2-04144] - CEM-T4 cell lysate. Western blot image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SFRS4 Antibody [NBP2-04144] - J-Lat cells treated with sh vectors targeting SR proteins were induced with Prostratin over 24 hours. Cells were harvested for protein using RIPA. Blots for SRSF4, GFP, HIV p24 GAG protein, and the stain free gel are shown. The J-LAt cell line used had an inducibe HIV construct that when induced would produce GFP as well as HIV proteins such as p24 (GAG). The sh vector was used to knock down the protein. Western blot image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SFRS4 Antibody [NBP2-04144] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SRp75 antibody NBP2-04144 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp75 was also immunoprecipitated by rabbit anti-SRp75 antibody NBP2-04143, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SFRS4 Antibody [NBP2-04144] - Sections of human lung carcinoma. Antibody: Affinity purified rabbit anti- SRp75 used at a dilution of 1:1000 (1 ug/mL). Detection: DAB. Counterstain: Hematoxylin (blue).
