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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-28548 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM83B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, MCF-7.
- Concentration
- 1 mg/mL
Submitted references MicroRNA‑199a/b‑5p inhibits endometrial cancer cell metastasis and invasion by targeting FAM83B in the epithelial‑to‑mesenchymal transition signaling pathway.
Family with sequence similarity 83, member B is a predictor of poor prognosis and a potential therapeutic target for lung adenocarcinoma expressing wild-type epidermal growth factor receptor.
Xiong H, Wang N, Chen H, Zhang M, Lin Q
Molecular medicine reports 2021 May;23(5)
Molecular medicine reports 2021 May;23(5)
Family with sequence similarity 83, member B is a predictor of poor prognosis and a potential therapeutic target for lung adenocarcinoma expressing wild-type epidermal growth factor receptor.
Yamaura T, Ezaki J, Okabe N, Takagi H, Ozaki Y, Inoue T, Watanabe Y, Fukuhara M, Muto S, Matsumura Y, Hasegawa T, Hoshino M, Osugi J, Shio Y, Waguri S, Tamura H, Imai JI, Ito E, Yanagisawa Y, Honma R, Watanabe S, Suzuki H
Oncology letters 2018 Feb;15(2):1549-1558
Oncology letters 2018 Feb;15(2):1549-1558
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAM83B using 30 µg of A549 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a FAM83B polyclonal antibody (Product # PA5-28548) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAM83B was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FAM83B Polyclonal Antibody (Product # PA5-28548) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAM83B was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a FAM83B Polyclonal Antibody (Product # PA5-28548) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using FAM83B (Product # PA5-28548) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Effect of miR-199a/b-5p/ FAM83B network on the EMT signaling pathway. (A) Western blot analysis indicated that transfection with miR-199a/b-5p mimics inhibited EMT signaling in the Ishikawa and JEC cells. The protein expression levels of FAM83B, N-cadherin, Snail, alpha-SMA, vimentin and Twist were downregulated, whereas those of E-cadherin were upregulated in the miR-199a/b-5p mimic-transfected cells. (B) Western blot analysis revealed that transfection with miR-199a/b-5p and FAM83B mimics activated the EMT signaling pathway in Ishikawa and JEC cells. The protein expression levels of FAM83B, N-cadherin, Snail, alpha-SMA, vimentin and Twist were upregulated, whereas those of E-cadherin were downregulated in the FAM83B mimic-transfected cells. **P
