HPA021911

antibody from Atlas Antibodies

Targeting: TRIM37 KIAA0898, MUL, POB1, TEF3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA021911

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA021911, RRID:AB_10964487

Product name

Anti-TRIM37

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

IHLLDLKDRSSIENLWGLQPRPPASLLQPTASYSR
KDKDQRKQQAMWRVPSDLKMLKRLKTQMAEVRCMK
TDVKNTLSEIKSSSAASGDMQTSLFSADQAALAAC
GTENSGRLQDLGMELLAKSS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines MCF-7 and SK-MEL-30 using Anti-TRIM37 antibody. Corresponding TRIM37 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.