10906-1-AP

antibody from Invitrogen Antibodies

Targeting: MYL2 CMH10

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10906-1-AP

Provider

Invitrogen Antibodies

Product name

Myosin Light Chain 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAPKKAKKR AGGANSNVFS MFEQTQIQEF KEAFTIMDQN RDGFIDKNDL RDTFAALGRV NVKNEEIDEM IKEAPGPINF TVFLTMFGEK LKGADPEETI LNAFKVFDPE GKGVLKADYV REMLTTQAER FSKEEVDQMF AAFPPDVTGN LDYKNLVHII THGEEKD (1-166 aa encoded by BC015821)

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10906-1-AP (Myosin Light Chain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10906-1-AP (Myosin Light Chain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat heart tissue were subjected to SDS PAGE followed by western blot with 10906-1-AP (Myosin Light Chain 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 10906-1-AP (Myosin Light Chain 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed mouse heart tissue using 10906-1-AP (Myosin Light Chain 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 10906-1-AP ( Myosin Light Chain 2 antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 10906-1-AP ( Myosin Light Chain 2 antibody at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using (Product # 10906-1-AP) (Myosin Light Chain 2/MLC-2V antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 10906-1-AP (Myosin Light Chain 2 antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 10906-1-AP (Myosin Light Chain 2 antibody at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Myosin Light Chain 2 (IP:10906-1-AP, 3ug; Detection:10906-1-AP 1:500) with mouse heart tissue lysate 6000ug.