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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-96616 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ASKAAAKTQE TNKETKTEAK EVTNASAAGN KAPGKGNMMS NFFGKAAMNK FKVNLDSEQA VKEEKIVEQP TVSVTEPKLA TPAGLKKSSK KAEPVKVLQK EKKRGKRVAL SDDETKETEN MRKKRRRIKL PESDSSEDEV FPDSPGAYEA ESPSPPPPPS PPLEPVPKTE PEPPSVKSSS GENKRKRKRV LKSKTYLDGE GCIVTEKVYE SESCTDSEEE LNMKTSSVHR PPAMTVKKEP REERKGPKKG TAALGKANRQ VSITGFFQRK; Positive Samples: THP-1, HepG2, HeLa, MCF7, H460, Mouse brain; Cellular Location: Nucleus
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.41 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of POLD3 in extracts of various cell lines using POLD3 Polyclonal Antibody (Product # PA5-96616) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of POLD3 was performed in GFP-RNF168 transgenic U2OS cells using POLD3 Polyclonal Antibody (Product # PA5-96616). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of POLD3 was performed in U2OS cells using POLD3 Polyclonal Antibody (Product # PA5-96616).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of POLD3 in paraffin-embedded human oophoroma using POLD3 Polyclonal Antibody (Product # PA5-96616) at a dilution of 1:100.
