Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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Validation data
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- Product number
- 21935-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLKDSGPLF NTDYDILKSN LQNCSKFSAI QCAAAVPRAP AESSSSSKKF EQSHLHMSSE TQANNELTTN GHGPPASKQV SQQPKGIMGM FASKAAAKTQ ETNKETKTEA KEVTNASAAG NKAPGKGNMM SNFFGKAAMN KFKVNLDSEQ AVKEEKIVEQ PTVSVTEPKL ATPAGLKKSS KKAEPVKVLQ KEKKRGKRVA LSDDETKETE NMRKKRRRIK LPESDSSEDE VFPDSPGAYE AESPSPPPPP SPPLEPVPKT EPEPPSVKSS SGENKRKRKR VLKSKTYLDG EGCIVTEKVY ESESCTDSEE ELNMKTSSVH RPPAMTVKKE PREERKGPKK GTAALGKANR QVSITGFFQR K (107-466 aa encoded by BC108908)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 21935-1-AP ( POLD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21935-1-AP (POLD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using 21935-1-AP (POLD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21935-1-AP (POLD3 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP ( POLD3 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21935-1-AP ( POLD3 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 21935-1-AP) (POLD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-POLD3 (IP:21935-1-AP, 4ug; Detection:21935-1-AP 1:300) with MCF-7 cells lysate 2560ug.