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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ7636 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GOT1 Antibody / Glutamic-oxaloacetic transaminase 1
- Antibody type
- Monoclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antibody clone number
- 6B3B4
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the GOT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human T-47D, 2) human HepG2, 3) human Caco-2, 4) rat brain, 5) rat liver, 6) mouse brain and 7) mouse liver tissue lysate with GOT1 antibody. Predicted molecular weight ~41 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human renal pelvis squamous tissue with GOT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with GOT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectum adenocarcinoma cancer tissue with GOT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HepG2 cells with GOT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GOT1 antibody.
