Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-109271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ORAI3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Orai3 in , Lane 1 : Raji cell lysates, Lane 2 : Raji cell lysates (anti-Orai3 antibody and immunizing peptide). Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Orai3 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai3 polyclonal antibody (Product # PA5-109271), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Orai3 in MG-63 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai3 polyclonal antibody (Product # PA5-109271), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Orai3 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai3 polyclonal antibody (Product # PA5-109271), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Orai3 in paraffin-embedded rat brain tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Orai3 in paraffin-embedded rat brain tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Orai3 in paraffin-embedded mouse testis tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Orai3 in paraffin-embedded human placenta tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Orai3 in paraffin-embedded human liver cancer tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Orai3 in paraffin-embedded human kidney tissue. Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Orai3 in A549 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Orai3 polyclonal antibody (Product # PA5-109271) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.