PAB27873

antibody from Abnova Corporation

Targeting: BRAP BRAP2, IMP, RNF52

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27873

Provider

Abnova Corporation

Product name

BRAP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant BRAP.

Antigen sequence

SLVVIRLELAEHSPVPAGFGFSAAAGEMSDEEIKK
TTLASAVACLEGKSPGEKVAIIHQHLGRREMTDVI
IETMKSNPDELKTTVEERKSSEA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with BRAP polyclonal antibody (Cat # PAB27873) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with BRAP polyclonal antibody (Cat # PAB27873) at 1-4 ug/mL dilution shows positivity in nuclear membrane & cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with BRAP polyclonal antibody (Cat # PAB27873) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)