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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- LS-C773828 - Provider product page
- Provider
- LSBio
- Product name
- TMEM49 Antibody (HRP) LS-C773828
- Antibody type
- Polyclonal
- Description
- Peptide Affinity Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Horseradish Peroxidase
- Storage
- Store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Human A549 showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A549 cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VMP1 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Vacuole Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VMP1 Antibody. (D) Composite.
