NBP2-78784

antibody from Novus Biologicals

Targeting: VMP1 EPG3, TANGO5, TMEM49

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-78784

Provider

Novus Biologicals

Product name

Rabbit Polyclonal TMEM49 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.7 mg/ml

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TMEM49 Antibody [NBP2-78784] - Total protein from human U2OS, HeLa, MCF7 and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TMEM49 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TMEM49 Antibody [NBP2-78784] - Analysis of a FFPE tissue section of the human testis using 1:200 dilution of TMEM49 antibody (NBP2-78784). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly strong cytoplasmic staining in cytoplasm and membrane in seminiferous tubules.