MA5-27547

antibody from Invitrogen Antibodies

Targeting: KCNN4 hIKCa1, hKCa4, hSK4, IK, KCa3.1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27547

Provider

Invitrogen Antibodies

Product name

KCNN4 (extracellular) Monoclonal Antibody (6C1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgM

Antibody clone number

6C1

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using 1) HEK cells transfected with control vector, and 2) HEK cells transfected with human KCa3.1 with KCNN4 (extracellular) polyclonal antibody (Product # MA5-27547) using a dilution of 1:250.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using 1) HEK cells transfected with control vector, and 2) HEK cells transfected with human KCa3.1 with KCNN4 (extracellular) polyclonal antibody (Product # MA5-27547) using a dilution of 1:250.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using 1) HEK cells transfected with control vector, and 2) HEK cells transfected with human KCa3.1 with KCNN4 (extracellular) polyclonal antibody (Product # MA5-27547) using a dilution of 1:250.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of KCNN4 (extracellular) in human LN-CaP prostate carcinoma cells. A) Samples were probed with KCNN4 (extracellular) monoclonal antibody (Product # MA5-27547) using a dilution of 1:20, and incubated with goat-anti-mouse-DyLight-594 (red). B) Live view of cells. C) Merged image of Panels A and B.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KCNN4 (extracellular) in mouse brain. A) Samples were probed with KCNN4 (extracellular) monoclonal antibody (Product # MA5-27547). Staining of KCa3.1 (red) is detected in neuroblasts.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of THP-1 cells using polyclonal KCNN4 (extracellular) antibody (Product # MA5-27547) with a dilution of 1:20 followed by a goat-anti-mouse-Cy5 secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of THP-1 cells using polyclonal KCNN4 (extracellular) antibody (Product # MA5-27547) with a dilution of 1:20 followed by a goat-anti-mouse-Cy5 secondary antibody.