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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-85076 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85076, RRID:AB_11027580
- Product name
- Rabbit Polyclonal DDX47 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat DDX47 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX47 Antibody [NBP1-85076] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX47 Antibody [NBP1-85076] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX47 Antibody [NBP1-85076] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
