Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 66046-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPS3 Monoclonal Antibody (2G7H4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAVQISKKR KFVADGIFKA ELNEFLTREL AEDGYSGVEV RVTPTRTEII ILATRTQNVL GEKGRRIREL TAVVQKRFGF PEGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH SGDPVNYYVD TAVRHVLLRQ GVLGIKVKIM LPWDPTGKIG PKKPLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA (1-243 aa encoded by BC003137)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G7H4
- Vial size
- 150 µL
- Concentration
- 1.12 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-IG (RPS3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 66046-1-IG (RPS3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 66046-1-Ig (RPS3 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 66046-1-Ig (RPS3 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 66046-1-Ig (RPS3 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 66046-1-Ig (RPS3 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 66046-1-IG ( RPS3 Antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RPS3 (IP:66046-1-IG, 3ug; Detection:66046-1-IG 1:2000) with HEK-293 cells lysate 1800ug.
