Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB2784 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CLEC4F/CLECSF13 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF4F, rhCLEC4D, rhCLEC4E, recombinant mouse (rm) OCIL, and rmOCIL-rp2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Lipopolysaccharide Lowers Cholesteryl Ester Transfer Protein by Activating F4/80(+)Clec4f(+)Vsig4(+)Ly6C(-) Kupffer Cell Subsets.
van der Tuin SJL, Li Z, Berbée JFP, Verkouter I, Ringnalda LE, Neele AE, van Klinken JB, Rensen SS, Fu J, de Winther MPJ, Groen AK, Rensen PCN, Willems van Dijk K, Wang Y
Journal of the American Heart Association 2018 Mar 10;7(6)
Journal of the American Heart Association 2018 Mar 10;7(6)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CLEC4F/CLECSF13 in Mouse Liver. CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse CLEC4F/CLECSF13 Monoclonal Antibody (Catalog # MAB2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.