H00001774-M02

antibody from Abnova Corporation

Targeting: DNASE1L1 DNAS1L1, DNASEX, DNL1L, XIB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001774-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001774-M02, RRID:AB_534842

Product name

DNASE1L1 monoclonal antibody (M02), clone 4E8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant DNASE1L1.

Antigen sequence

AFRICAFNAQRLTLAKVAREQVMDTLVRILARCDI
MVLQEVVDSSGSAIPLLLRELNRFDGSGPYSTLSS
PQLGRSTYMETYVYFYRSHKTQVLSSYVYNDEDDV
FAREPFVAQFSLPSNVLPSLVLVPLHTTPKAVEKE
LNALYDVFLEVSQHWQSKDVILLGDFNADCASLTK
KRLDKLELRTEPGFHWVIADGEDTTVRASTHCTYD
RVVLHGERCRSLLHTAAAFDFPTSFQLTEEEALNI
SDHYPVEVELKLSQAHSVQPLSLTVLLLLSLLSPQ
LCPAA


Isotype

IgG

Antibody clone number

4E8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DNASE1L1 expression in transfected 293T cell line by DNASE1L1 monoclonal antibody (M02), clone 4E8.Lane 1: DNASE1L1 transfected lysate(33.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DNASE1L1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol