Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-13124 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRY1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Core circadian clock gene expression in human dental pulp-derived cells in response to L-mimosine, hypoxia and echinomycin.
Astrocyte deletion of Bmal1 alters daily locomotor activity and cognitive functions via GABA signalling.
Janjić K, Kurzmann C, Moritz A, Agis H
European journal of oral sciences 2018 Aug;126(4):263-271
European journal of oral sciences 2018 Aug;126(4):263-271
Astrocyte deletion of Bmal1 alters daily locomotor activity and cognitive functions via GABA signalling.
Barca-Mayo O, Pons-Espinal M, Follert P, Armirotti A, Berdondini L, De Pietri Tonelli D
Nature communications 2017 Feb 10;8:14336
Nature communications 2017 Feb 10;8:14336
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cry1 using a Cry1 polyclonal antibody (Product # PA5-13124) in placenta tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cry1 using a Cry1 polyclonal antibody (Product # PA5-13124) in placenta tissue lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a Cry1 polyclonal antibody (Product # PA5-13124), followed by HRP-conjugated secondary antibody and AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Bmal1 knockdown in astrocytes suppresses entrainment of co-cultured cortical neurons in vitro . ( a ) Primary cortical astrocytes were transfected with scramble (Scrbl) or Bmal1 siRNAs. After 48 h, astrocytes were synchronized with 100 nM of Dexamethasone for 2 h (Astro Dexa). After washing, astrocytes were placed in co-culture with asynchronous cortical neurons without physical contact, but sharing the same culture media. ( b ) Bmal1 , Cry1 , Per2 and BMAL1 target Dbp were analysed in astrocytes (upper panels) and neurons (lower panels) at the indicated time points by quantitative PCR. Graphs show the mean+-s.e.m. of the cosine-fitted curves from three experiments performed in triplicate. ( c ) Representative images of western blottings for BMAL1 in primary astrocytes (left panels) or CRY1 in co-cultured neurons (right panels), showing expression of BMAL1 in Dexamethasone-treated astrocytes in isolated cultures (top) or Dexamethasone-treated astrocytes in co-culture with asynchronous neurons (middle and bottom), on transfection with scramble siRNAs (CSA Scrbl) or on transfection with Bmal1 siRNAs (CSA KD); (right panels) entrainment of CRY1 in cortical neurons after co-culture with Scrbl transfected synchronous astrocytes (Neu-CSA Scrbl) is not observed when co-culture is performed with arrhythmic astrocytes (Neu-CSA KD) ( n =2 independent experiments).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Effect of L-mimosine (L- MIM ), hypoxia and echinomycin on the levels of core clock proteins in two-dimensional (2D) monolayer and three-dimensional (3D) spheroid cultures of dental pulp-derived cells ( DPC ). The DPC in 2D monolayer cultures were treated with L- MIM or hypoxia (A) and with L- MIM or hypoxia in combination with echinomycin or with echinomycin alone (C) for 24 h. Further DPC were cultured in 3D spheroid cultures and treated with L- MIM or hypoxia (B). The amounts of circadian locomotor output cycles kaput ( CLOCK ), cryptochrome circadian regulator 1 and 2 ( CRY 1 and CRY 2, respectively) and period circadian regulator 3 ( PER 3) proteins produced were detected by western blotting. Glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) was used as reference protein. Experiments were conducted at least twice with DPC from two different donors ( n = 4).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Core clock gene mRNA and protein levels under normoxia, L-mimosine (L- MIM ) and hypoxia change during the observation period. Dental pulp-derived cells ( DPC ) in two-dimensional (2D) monolayer cultures were serum-starved and afterwards treated with L- MIM or hypoxia. mRNA (A-D) and protein (E-I) levels of circadian locomotor output cycles kaput ( CLOCK ), cryptochrome circadian regulator 1 and 2 ( CRY 1 and CRY 2, respectively) and period circadian regulator 3 ( PER 3) were measured in a 4-h interval over 48 h by quantitative PCR ( qPCR ) and western blotting, respectively. mRNA levels are displayed relative to glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) and 0 h after serum starvation (A-D). GAPDH was used as reference protein (E-I). Experiments were conducted at least twice with DPC from two different donors ( n = 4).
