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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6504 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ORC5L Antibody
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ORC5L antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) monkey COS-7, 2) human placenta, 3) human K562, 4) human MCF-7, 5) rat testis, 6) rat kidney, 7) mouse ovary and 8) mouse testis lysate with ORC5L antibody. Predicted molecular weight ~50 kDa (isoform 1), ~37 kDa (isoform 2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human MCF-7 cells with ORC5L antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with ORC5L antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ORC5L antibody.
