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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-102480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APOC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total APOC2.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Hepatocyte Small Heterodimer Partner Mediates Sex-Specific Effects on Triglyceride Metabolism via Androgen Receptor in Male Mice.
Palmisano BT, Zhu L, Litts B, Burman A, Yu S, Neuman JC, Anozie U, Luu TN, Edington EM, Stafford JM
Metabolites 2021 May 20;11(5)
Metabolites 2021 May 20;11(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APOC2 in mouse liver tissue lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with APOC2 polyclonal antibody (Product # PA5-102480).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded APOC2 in mouse liver tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with APOC2 polyclonal antibody (Product # PA5-102480) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Effect of hepatic deletion of SHP on lipoprotein lipase activities. ( A - F ) Plasma ApoC1, ApoC2, ApoC3, ApoE, and ApoA1 levels were determined with immunoblotting and blot quantifications. ( G ) Liver mRNA levels of AR in intact or GDX fl/fl and SHP Deltahep mice. ( H , I ) Plasma from chow ( H ) and fructose ( I ) fed mice was used to evaluate lipoprotein lipase activity. Data shown are mean +- SD ( n = 6~8/group), # p < 0.05 and ## p < 0.01 for genotype effect, and $$ p < 0.01 and $$$ p < 0.001 for treatment effects (2-way ANOVA). * p < 0.05 ( t -test).
