NBP2-32114

antibody from Novus Biologicals

Targeting: CLPTM1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-32114

Provider

Novus Biologicals

Product name

Rabbit Polyclonal CLPTM1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CLPTM1 Antibody [NBP2-32114] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-CLPTM1 antibody NBP2-32114) used for IP at 6 ug per reaction. CLPTM1 was also immunoprecipitated by rabbit anti-CLPTM1 antibody BL16525. For blotting immunoprecipitated CLPTM1, NBP2-32114was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CLPTM1 Antibody [NBP2-32114] - Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CLPTM1 antibody NBP2-32114 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CLPTM1 Antibody [NBP2-32114] - Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CLPTM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.