ALX-215-001-R100

antibody from Enzo Life Sciences

Targeting: MAPT DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, PPP1R103, tau

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ALX-215-001-R100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-215-001-R100, RRID:AB_2050501

Product name

[pSer262]Tau polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Epitope affinity-purified. Negatively preadsorbed by non-phosphorylated immunogen peptide.

Reactivity

Human

Host

Rabbit

Vial size

100 μl

Storage

-20°C

Handling

For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.

References

Submitted references

Dephosphorylation of tau by protein phosphatase 5: impairment in Alzheimer's disease.

Liu F, Iqbal K, Grundke-Iqbal I, Rossie S, Gong CX
The Journal of biological chemistry 2005 Jan 21;280(3):1790-6

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Involvement of aberrant glycosylation in phosphorylation of tau by cdk5 and GSK-3beta.

Liu F, Iqbal K, Grundke-Iqbal I, Gong CX
FEBS letters 2002 Oct 23;530(1-3):209-14

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Role of glycosylation in hyperphosphorylation of tau in Alzheimer's disease.

Liu F, Zaidi T, Iqbal K, Grundke-Iqbal I, Merkle RK, Gong CX
FEBS letters 2002 Feb 13;512(1-3):101-6

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Peptide competition. Only the phosphopetide corresponding to tau [pS262] blocks the antibody signal, demonstrating the specificity of the antibody. Tau [pS262] phophorylation is induced by the addition of PKA. Method: Human recombinant tau untreated (lane 1) or treated with PKA (36µg per µg tau) for 45 min and added to background extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4¡C, then incubated with PAb to Tau (phosphorylated) (pSer262) (Prod. No. ALX-215-001) for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignalTM method.