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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP31562PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP31562PU-N, RRID:AB_10981032
- Product name
- anti Mast Cell Chymase
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence C-QFRHPKYNTSTLHHD, from the internal region of the protein sequence according to NP_001827.1.
- Reactivity
- Human
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0,5 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 lysate (10µg protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with this antibody (AP31562PU-N)(0.5µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with this antibody (AP31562PU-N)(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
