Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 18189-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mast Cell Chymase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLLLPLPLL LFLLCSRAEA GEIIGGTECK PHSRPYMAYL EIVTSNGPSK FCGGFLIRRN FVLTAAHCAG RSITVTLGAH NITEEEDTWQ KLEVIKQFRH PKYNTSTLHH DIMLLKLKEK ASLTLAVGTL PFPSQFNFVP PGRMCRVAGW GRTGVLKPGS DTLQEVKLRL MDPQACSHFR DFDHNLQLCV GNPRKTKSAF KGDSGGPLLC AGVAQGIVSY GRSDAKPPAV FTRISHYRPW INQILQAN (1-247 aa encoded by BC069370)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using (Product # 18189-1-AP) (Mast Cell Chymase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Mast Cell Chymase (IP:18189-1-AP, 4ug; Detection:18189-1-AP 1:300) with mouse heart tissue lysate 2400ug.
