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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- MA5-11717 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mast Cell Chymase Monoclonal Antibody (CC1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-11717 targets Mast Cell Chymase in IHC (P) and WB applications and shows reactivity with Human samples.
- Antibody clone number
- CC1
- Concentration
- 80 µg/mL
Submitted references The role of macrophage migration inhibitory factor in mast cell-stimulated fibroblast proliferation and collagen production.
Angiotensin AT2 receptor agonist prevents salt-sensitive hypertension in obese Zucker rats.
Increased plasma chymase concentration and mast cell chymase expression in venous neointimal lesions of patients with CKD and ESRD.
Hyperexpression of the high-affinity IgE receptor-beta chain in chronic allergic keratoconjunctivitis.
Identification of mast cells and mast cell subpopulations.
Immediate anaphylactic death following antibiotics injection: splenic eosinophilia easily revealed by pagoda red stain.
Interstitial Cajal-like cells in rat mesentery: an ultrastructural and immunohistochemical approach.
Cannabinoids reduce granuloma-associated angiogenesis in rats by controlling transcription and expression of mast cell protease-5.
Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells.
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors.
c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases.
Ningyan G, Xu Y, Hongfei S, Jingjing C, Min C
PloS one 2015;10(3):e0122482
PloS one 2015;10(3):e0122482
Angiotensin AT2 receptor agonist prevents salt-sensitive hypertension in obese Zucker rats.
Ali Q, Patel S, Hussain T
American journal of physiology. Renal physiology 2015 Jun 15;308(12):F1379-85
American journal of physiology. Renal physiology 2015 Jun 15;308(12):F1379-85
Increased plasma chymase concentration and mast cell chymase expression in venous neointimal lesions of patients with CKD and ESRD.
Wasse H, Rivera AA, Huang R, Martinson DE, Long Q, McKinnon W, Naqvi N, Husain A
Seminars in dialysis 2011 Nov-Dec;24(6):688-93
Seminars in dialysis 2011 Nov-Dec;24(6):688-93
Hyperexpression of the high-affinity IgE receptor-beta chain in chronic allergic keratoconjunctivitis.
Matsuda A, Okayama Y, Ebihara N, Yokoi N, Hamuro J, Walls AF, Ra C, Hopkin JM, Kinoshita S
Investigative ophthalmology & visual science 2009 Jun;50(6):2871-7
Investigative ophthalmology & visual science 2009 Jun;50(6):2871-7
Identification of mast cells and mast cell subpopulations.
Buckley M, Walls AF
Methods in molecular medicine 2008;138:285-97
Methods in molecular medicine 2008;138:285-97
Immediate anaphylactic death following antibiotics injection: splenic eosinophilia easily revealed by pagoda red stain.
Trani N, Bonetti LR, Gualandri G, Barbolini G
Forensic science international 2008 Oct 25;181(1-3):21-5
Forensic science international 2008 Oct 25;181(1-3):21-5
Interstitial Cajal-like cells in rat mesentery: an ultrastructural and immunohistochemical approach.
Hinescu ME, Popescu LM, Gherghiceanu M, Faussone-Pellegrini MS
Journal of cellular and molecular medicine 2008 Jan-Feb;12(1):260-70
Journal of cellular and molecular medicine 2008 Jan-Feb;12(1):260-70
Cannabinoids reduce granuloma-associated angiogenesis in rats by controlling transcription and expression of mast cell protease-5.
De Filippis D, Russo A, D'Amico A, Esposito G, Pietropaolo C, Cinelli M, Russo G, Iuvone T
British journal of pharmacology 2008 Aug;154(8):1672-9
British journal of pharmacology 2008 Aug;154(8):1672-9
Anti-CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells.
Fukiwake N, Moroi Y, Imafuku S, Masuda T, Kokuba H, Furue M, Urabe K
The Journal of dermatology 2006 May;33(5):326-30
The Journal of dermatology 2006 May;33(5):326-30
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Popescu LM, Gherghiceanu M, Hinescu ME, Cretoiu D, Ceafalan L, Regalia T, Popescu AC, Ardeleanu C, Mandache E
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
Popescu LM, Gherghiceanu M, Hinescu ME, Cretoiu D, Ceafalan L, Regalia T, Popescu AC, Ardeleanu C, Mandache E
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Journal of cellular and molecular medicine 2006 Apr-Jun;10(2):429-58
Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors.
Fernandez NJ, West KH, Jackson ML, Kidney BA
Veterinary pathology 2005 Jul;42(4):437-45
Veterinary pathology 2005 Jul;42(4):437-45
c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases.
Edwards ST, Cruz AC, Donnelly S, Dazin PF, Schulman ES, Jones KD, Wolters PJ, Hoopes C, Dolganov GM, Fang KC
The Journal of pathology 2005 Jul;206(3):279-90
The Journal of pathology 2005 Jul;206(3):279-90
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with Mast Cell Chymase antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 Characterization of mast cells derived from bone marrow CD34+ hematopoietic precursors. A. Immunofluorescence staining of original CD34+ hematopoietic precursors and differentiated mast cells. Anti-tryptase antibody or anti-chymase antibody was used in the experiments. DAPI was used to stain the nucleus of the cells. B. Quantification of experiments described in A, more than 200 cells were counted in each group, mean+-SD, n = 3. C. Toluidine blue staining of the derived mast cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 5 MIF knockdown inhibits the profibrogenic activity of Mast cells. A and B. The mRNA and protein levels of MIF in mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. C. Western-blots of chymase, tryptase and GAPDH in original or mast cells (derived from CD34+ hematopoietic precursors with or without MIF knockdown). D. Toluidine blue staining of the mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. E. Quantification analysis of FcepsilonRI/CD117 double positive population in original or mast cells (derived from CD34+ hematopoietic precursors with or without MIF knockdown), #P>0.05. F. Histamine release assay of the mast cells derived from CD34+ hematopoietic precursors with or without MIF knockdown. G and H. The growth curves and collagen synthesis of fibroblast cultured in medium conditioned by mast cells with or without MIF knockdown, mean+-SD, n = 3.
