Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PB9086 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Integrin beta 1/ITGB1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Integrin beta 1/ITGB1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Integrin beta 1/ITGB1 information: Molecular Weight: 88415 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Cell projection, invadopodium membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single- pass type I membrane protein . Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid- telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner; Tissue Specificity: Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non- proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Fibronectin binds integrin α5β1 to regulate macular neovascularization through the Wnt/β-catenin signaling pathway.
Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.
Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype.
Inhibition of the peritoneal metastasis of human gastric cancer cells by dextran sulphate in vivo and in vitro.
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC‑α/ERK1/2 pathway: An in vitro investigation.
Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-α-ERK1/2-NF-κB pathway.
Mechanical stiffness of liver tissues in relation to integrin β1 expression may influence the development of hepatic cirrhosis and hepatocellular carcinoma.
Zhao X, Hu L, Liu G, Yin X, Gong Q, Li Y, Li Q, Zhou Y, Sun Y, Guo C, Du Z
Experimental eye research 2024 May;242:109880
Experimental eye research 2024 May;242:109880
Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.
Zhang B, Fan M, Fan J, Luo Y, Wang J, Wang Y, Liu B, Sun Y, Zhao Q, Hiscox JA, Nan Y, Zhou EM
Microbiology spectrum 2022 Feb 23;10(1):e0226521
Microbiology spectrum 2022 Feb 23;10(1):e0226521
Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype.
Yang S, Sun Y, Geng Z, Ma K, Sun X, Fu X
International journal of molecular medicine 2016 May;37(5):1263-73
International journal of molecular medicine 2016 May;37(5):1263-73
Inhibition of the peritoneal metastasis of human gastric cancer cells by dextran sulphate in vivo and in vitro.
Xu Y, Huang Y, Wang H, Liu Y
Oncology letters 2016 Apr;11(4):2384-2390
Oncology letters 2016 Apr;11(4):2384-2390
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC‑α/ERK1/2 pathway: An in vitro investigation.
Cheng XD, Gu JF, Yuan JR, Feng L, Jia XB
Molecular medicine reports 2015 Dec;12(6):7992-8002
Molecular medicine reports 2015 Dec;12(6):7992-8002
Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-α-ERK1/2-NF-κB pathway.
Cheng X, Gu J, Zhang M, Yuan J, Zhao B, Jiang J, Jia X
International immunopharmacology 2014 Nov;23(1):304-13
International immunopharmacology 2014 Nov;23(1):304-13
Mechanical stiffness of liver tissues in relation to integrin β1 expression may influence the development of hepatic cirrhosis and hepatocellular carcinoma.
Zhao G, Cui J, Qin Q, Zhang J, Liu L, Deng S, Wu C, Yang M, Li S, Wang C
Journal of surgical oncology 2010 Oct 1;102(5):482-9
Journal of surgical oncology 2010 Oct 1;102(5):482-9
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti-ITGB1 Picoband antibody, PB9086-3.jpgAll lanes: Anti-ITGB1(PB9086) at 0.5ug/mlWB: Hela Whole Cell Lysate at 40ugPredicted bind size: 88KDObserved bind size: 130KD
- Additional image