NBP2-36561

antibody from Novus Biologicals

Targeting: ITGB1 CD29, FNRB, GPIIA, MDF2, MSK12

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NBP2-36561

Provider

Novus Biologicals

Product name

Mouse Monoclonal Integrin beta 1/CD29 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Reacts with human Integrin beta 1. Epitope is not trypsin sensitive.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion.

Pinnington SJL, Marshall JF, Wheeler AP
Methods in molecular biology (Clifton, N.J.) 2018;1764:253-265

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PAG1 promotes the inherent radioresistance of laryngeal cancer cells via activation of STAT3.

Shen L, Ke Q, Chai J, Zhang C, Qiu L, Peng F, Deng X, Luo Z
Experimental cell research 2018 Sep 1;370(1):127-136

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Acidic pHe regulates cytoskeletal dynamics through conformational integrin β1 activation and promotes membrane protrusion.

Li S, Xiong N, Peng Y, Tang K, Bai H, Lv X, Jiang Y, Qin X, Yang H, Wu C, Zhou P, Liu Y
Biochimica et biophysica acta. Molecular basis of disease 2018 Jul;1864(7):2395-2408

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A Disintegrin and Metalloprotease-17 Regulates Pressure Overload-Induced Myocardial Hypertrophy and Dysfunction Through Proteolytic Processing of Integrin β1.

Fan D, Takawale A, Shen M, Samokhvalov V, Basu R, Patel V, Wang X, Fernandez-Patron C, Seubert JM, Oudit GY, Kassiri Z
Hypertension (Dallas, Tex. : 1979) 2016 Oct;68(4):937-48

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Integrin/Fak/Src-mediated regulation of cell survival and anoikis in human intestinal epithelial crypt cells: selective engagement and roles of PI3-K isoform complexes.

Beauséjour M, Noël D, Thibodeau S, Bouchard V, Harnois C, Beaulieu JF, Demers MJ, Vachon PH
Apoptosis : an international journal on programmed cell death 2012 Jun;17(6):566-78

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Integrin alpha(v)beta1 is an adenovirus coreceptor.

Li E, Brown SL, Stupack DG, Puente XS, Cheresh DA, Nemerow GR
Journal of virology 2001 Jun;75(11):5405-9

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Integrin expression and function on human osteoblast-like cells.

Gronthos S, Stewart K, Graves SE, Hay S, Simmons PJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 1997 Aug;12(8):1189-97

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An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice.

Mitjans F, Sander D, Adán J, Sutter A, Martinez JM, Jäggle CS, Moyano JM, Kreysch HG, Piulats J, Goodman SL
Journal of cell science 1995 Aug;108 ( Pt 8):2825-38

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Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies.

Takada Y, Puzon W
The Journal of biological chemistry 1993 Aug 15;268(23):17597-601

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A monoclonal antibody to beta 1 integrin (CD29) stimulates VLA-dependent adherence of leukocytes to human umbilical vein endothelial cells and matrix components.

Kovach NL, Carlos TM, Yee E, Harlan JM
The Journal of cell biology 1992 Jan;116(2):499-509

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).