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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [2]
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- Product number
- NB100-63255 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-63255, RRID:AB_960088
- Product name
- Mouse Monoclonal Integrin beta 1/CD29 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Epitope appears to be located aroun
d residues 207-218 in the b1 subuni
t putative A-domain.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated α(1)β(1) Integrin-Mediated Motility.
Wu D, Witt RL, Harrington DA, Farach-Carson MC
Frontiers in cell and developmental biology 2019;7:224
Frontiers in cell and developmental biology 2019;7:224
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD29.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.
