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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
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- Product number
- AF4075 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Plexin A3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Plexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, and rhPlexin D1 is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P70208- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
Demyanenko GP, Mohan V, Zhang X, Brennaman LH, Dharbal KE, Tran TS, Manis PB, Maness PF
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 20;34(34):11274-87
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 20;34(34):11274-87
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Plexin A3 in Rat Spinal Cord. Plexin A3 was detected in immersion fixed frozen sections of embryonic rat spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Plexin A3 in Mouse Spinal Cord. Plexin A3 was detected in immersion fixed frozen sections of mouse spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
