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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AB0005-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0005-200, RRID:AB_2333135
- Product name
- Anti-EEA1
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Endogenous EEA1 detected with AB0005 Ab at 1/500 dilution; lysate at 100 µg per lane
- Sample type
- At-T20
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Immunostaining of Hepa1-6 cells with EEA1 antibody (AB0005) at 1/50 dilution. Cells were fixed with 4% PFA
- Sample type
- Hepa1-6 cells
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated
