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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-33585 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-33585, RRID:AB_2277714
- Product name
- Anti-EEA1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references The helminth product ES-62 protects against septic shock via Toll-like receptor 4–dependent autophagosomal degradation of the adaptor MyD88
MUC1 regulates nuclear localization and function of the epidermal growth factor receptor.
Investigation of the biophysical and cell biological properties of ferroportin, a multipass integral membrane protein iron exporter.
Padmam Puneet, Mairi A McGrath, Hwee Kee Tay, Lamyaa Al-Riyami, Justyna Rzepecka, Shabbir M Moochhala, Shazib Pervaiz, Margaret M Harnett, William Harnett, Alirio J Melendez
Nature Immunology 2011 Feb;12(4):344-351
Nature Immunology 2011 Feb;12(4):344-351
MUC1 regulates nuclear localization and function of the epidermal growth factor receptor.
Bitler BG, Goverdhan A, Schroeder JA
Journal of cell science 2010 May 15;123(Pt 10):1716-23
Journal of cell science 2010 May 15;123(Pt 10):1716-23
Investigation of the biophysical and cell biological properties of ferroportin, a multipass integral membrane protein iron exporter.
Rice AE, Mendez MJ, Hokanson CA, Rees DC, Björkman PJ
Journal of molecular biology 2009 Feb 27;386(3):717-32
Journal of molecular biology 2009 Feb 27;386(3):717-32
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 162
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human hippocampus shows strong cytoplasmic and nuclear positivity in neuronal cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
