GTX109638

antibody from GeneTex

Targeting: EEA1 ZFYVE2

Provider product page for GTX109638

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [9]
Comments [0]
Validations
Western blot [4]
Immunocytochemistry [2]
Immunohistochemistry [3]

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Product number: GTX109638 - Provider product page
Provider: GeneTex
Proper citation: GeneTex Cat#GTX109638, RRID:AB_1950162
Product name: EEA1 antibody [C3], C-term
Antibody type: Polyclonal
Reactivity: Human, Mouse, Rat, Hamster
Host: Rabbit

EEA1 protein structure - GTX109638 shown in red.

Submitted references Laminin-1 induces endocytosis of 67KDa laminin receptor and protects Neuroscreen-1 cells against death induced by serum withdrawal.

Gopalakrishna R, Gundimeda U, Zhou S, Bui H, Davis A, McNeill T, Mack W
Biochemical and biophysical research communications 2018 Jan 1;495(1):230-237

Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.

Lai JH, Wang MY, Huang CY, Wu CH, Hung LF, Yang CY, Ke PY, Luo SF, Liu SJ, Ho LJ
EMBO reports 2018 Aug;19(8)

Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells.

Chen NC, Chyau CC, Lee YJ, Tseng HC, Chou FP
Scientific reports 2016 Feb 3;6:20417

Honokiol, a Lignan Biphenol Derived from the Magnolia Tree, Inhibits Dengue Virus Type 2 Infection.

Fang CY, Chen SJ, Wu HN, Ping YH, Lin CY, Shiuan D, Chen CL, Lee YR, Huang KJ
Viruses 2015 Sep 10;7(9):4894-910

The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.

Chou SF, Tsai ML, Huang JY, Chang YS, Shih C
PLoS pathogens 2015 Oct;11(10):e1005123

Astroglial IFITM3 mediates neuronal impairments following neonatal immune challenge in mice.

Ibi D, Nagai T, Nakajima A, Mizoguchi H, Kawase T, Tsuboi D, Kano S, Sato Y, Hayakawa M, Lange UC, Adams DJ, Surani MA, Satoh T, Sawa A, Kaibuchi K, Nabeshima T, Yamada K
Glia 2013 May;61(5):679-93

Cadmium-based quantum dot induced autophagy formation for cell survival via oxidative stress.

Luo YH, Wu SB, Wei YH, Chen YC, Tsai MH, Ho CC, Lin SY, Yang CS, Lin P
Chemical research in toxicology 2013 May 20;26(5):662-73

R26R-GR: a Cre-activable dual fluorescent protein reporter mouse.

Chen YT, Tsai MS, Yang TL, Ku AT, Huang KH, Huang CY, Chou FJ, Fan HH, Hong JB, Yen ST, Wang WL, Lin CC, Hsu YC, Su KY, Su IC, Jang CW, Behringer RR, Favaro R, Nicolis SK, Chien CL, Lin SW, Yu IS
PloS one 2012;7(9):e46171

Receptor chimeras demonstrate that the C-terminal domain of the human cytomegalovirus US27 gene product is necessary and sufficient for intracellular receptor localization.

Stapleton LK, Arnolds KL, Lares AP, Devito TM, Spencer JV
Virology journal 2012 Feb 16;9:42

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX109638 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract 5% SDS-PAGEEEA1 antibody [C3], C-term (GTX109638) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30?g whole cell lysate)A: 293T B: A431 (GTX27909)C: H1299 D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)5% SDS PAGEGTX109638 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.A. 30 ?g HepG2 whole cell lysate/extract B. 30 ?g A375 whole cell lysate/extract5% SDS-PAGEEEA1 antibody [C3], C-term (GTX109638) dilution: 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1(GTX109638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: EEA1 protein stained by EEA1 antibody [C3], C-term (GTX109638) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.